Journal: Journal of neurovirology

Article Title: Impaired neurogenesis and neurite outgrowth in an HIV-gp120 transgenic model is reversed by exercise via BDNF production and Cdk5 regulation

doi: 10.1007/s13365-013-0194-6

Figure Lengend Snippet: Shown are sample immunoreactive bands from Western blots. Lysates from the entire brain or the hippocampus of wild type and gp120 transgenic mice were subjected to Western blot analysis for brain-derived neurotrophic factor (BDNF, 15 kDa) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, as an internal control, 35 kDa). The ratio of BDNF/GAPDH was quantified. Values represent mean ± SEM (n = 3 animals in each group; *p < 0.05; **p < 0.01; ***p < 0.001, one-way ANOVA followed by Duncan's test). A and B: BDNF expression was down-regulated in the entire brain and the hippocampus of gp120 transgenic mice. C and D: Hippocampal BDNF levels were increased in gp120 transgenic mice after running for 10 days. E: Hippocampal BDNF levels were increased by running for 10 or 20 days, but decreased by detraining in both wild type and gp120 transgenic mice. F: There was a positive correlation between the expression level of hippocampal BDNF and running distance.

Article Snippet: The following primary antibodies were used: anti- brain derived neurotrophic factor (BDNF) (rabbit, 1:500, Santa Cruz Biotechnology) and anti-p35 (rabbit, 1:500, Santa Cruz Biotechnology).

Techniques: Western Blot, Transgenic Assay, Derivative Assay, Expressing

Journal: Journal of neurovirology

Article Title: Impaired neurogenesis and neurite outgrowth in an HIV-gp120 transgenic model is reversed by exercise via BDNF production and Cdk5 regulation

doi: 10.1007/s13365-013-0194-6

Figure Lengend Snippet: A: Representative photomicrographs demonstrate immunostaining of beta-III tubulin-positive (red) neurons in human fetal-derived neural precursor cells (fNPCs) under different conditions. fNPCs were transfected with a GFP expression plasmid or co-transfected with plasmids encoding p25 and Cdk5. The transfected cells were then exposed to recombinant gp120 protein (30 pM) with or without recombinant BDNF (100 ng/ml) for 24, 48 or 96 hours. Cells were immunostained using anti-beta-III tubulin (red). Scale bar = 200 µm. B: Quantitative analysis of neurite length demonstrated that gp120 exposure resulted in an increase in neurite outgrowth in a time-dependent manner by a similar amount compared with that in fNPCs co-transfected with p25 and Cdk5. BDNF treatment completely abolished gp120-induced neurite outgrowth. Values represent mean ± SEM (*p < 0.05; **p < 0.01; ***p < 0.001, one-way ANOVA followed by Duncan's test).

Article Snippet: The following primary antibodies were used: anti- brain derived neurotrophic factor (BDNF) (rabbit, 1:500, Santa Cruz Biotechnology) and anti-p35 (rabbit, 1:500, Santa Cruz Biotechnology).

Techniques: Immunostaining, Derivative Assay, Transfection, Expressing, Plasmid Preparation, Recombinant

Journal: Bioengineered

Article Title: Effect and mechanism of BDNF/TrkB signaling on vestibular compensation

doi: 10.1080/21655979.2021.1997565

Figure Lengend Snippet: List of primary antibodies used for western blots

Article Snippet: BDNF , Rabbit Anti-BDNF antibody , ab108319 , Abcam , 1:1000.

Techniques: Western Blot